Scan
Wild / mixed culture

Lactobacillus

The fast-souring bacteria of Berliner Weisse, gose, and modern kettle sours. Produces clean lactic tartness.

Also known as:Lacto · L. brevis · L. plantarum · L. delbrueckii · Sour worting bacteria
Category
Wild / mixed culture
Attenuation
Low — Lacto consumes ~5-10% of sugars before being killed by the boil
Flocculation
N/A — boiled off after souring
ABV Tolerance
Low — most strains die above 5% ABV

What it tastes like

Lactobacillus is the bacteria that turns sweet wort into sour wort. Different species produce different character — L. plantarum is fast and clean (24-48 hours to a 3.3 pH), L. brevis is slower with more complex character, L. delbrueckii is the traditional Berliner Weisse and lambic acidifier. Most modern American kettle sours use L. plantarum because it works fast and doesn't add weird flavors. After souring, you boil the wort (killing the Lacto), then ferment with regular ale yeast. Two-organism beer in one batch.

clean lactic tartnessyogurt-like (in concentrated form)no funk characterlemon brightness when fermented with citrus hops

Best in these styles

Fermentation profile

Kettle souring procedure: mash and lauter as normal, briefly boil to kill background bugs, cool to 95-110°F, pitch Lacto (commercial pure culture OR a handful of crushed grain for the wild approach), purge with CO2 or top with plastic wrap to prevent oxidation (which produces vinegar). Hold 24-72 hours until pH hits target (3.2-3.4). Then boil to kill Lacto, add hops, ferment with regular ale yeast.

Temp range
85-115°F (29-46°C) — yes, that warm
Ideal temp
95-110°F (kettle souring)
Esters
None — produces lactic acid, not ester compounds
What to avoid
Oxygen exposure during the souring phase. Lacto in the presence of oxygen produces acetic acid (vinegar) instead of lactic acid. Cover the kettle completely. Also: don't add hops before the Lacto phase — most Lacto strains are inhibited by even small amounts of hop alpha acids (low-IBU strains exist but the simpler path is to add hops after).

Available as

Lactobacillus is sold under multiple supplier brand names — same or near-identical strain.

FormatSupplierProduct codeNotes
Liquid White Labs WLP677 L. delbrueckii 100B cells
Traditional Berliner Weisse strain
Liquid Omega OYL-605 Lacto Blend Various
Mixed Lacto strains for fast clean souring
Liquid Wyeast 5335 L. buchneri 100B cells
Powder Goodbelly probiotic shot L. plantarum (Probiotic from grocery store) 2.7oz bottle
Cheap, accessible, works — homebrewer trick

Comparable strains

If you can't source this strain, these alternatives bring overlapping character or fermentation behavior.

History

Lactobacillus has been souring beer for thousands of years — every wild fermentation of grain has some Lacto activity. The technique was largely abandoned in modern brewing after Pasteur's discoveries on sterile fermentation in the 1800s. Sour beer's revival in the 2000s (driven by Russian River, Cantillon, etc.) brought Lacto back. The modern 'kettle sour' technique — fast, controlled Lacto souring before primary fermentation — was popularized by US craft breweries in the early 2010s and is now standard for fruited and citrus-driven American sours.

Related brewing guides

← Back to yeast strain guide